Talks and Poster Presentations (without Proceedings-Entry):

F. Trognitz, A. Burg, T. Grahsl, B. Trognitz:
"Diversity of resistance genes, their analogs, and genes involved in plant-microbe interactions.";
Talk: In: Proc. 1st Solanaceae Genome Workshop, Sept. 19-21, 2004, Wageningen, The Netherlands. A meeting by the Intl. Solanaceae Genome Sequencing Consortium (PGSC): P9-22. ( http://www.solanaceae2004.org/ ), Wageningen/netherlands; 07-11-2004 - 07-16-2004.



English abstract:
Abstract : The resistance (R) proteins of the TIR- and non-TIR (carrying a coiled coil sequence at their N terminus) superfamilies possess a nucleotide binding site (NBS) domain that fulfills essential functions in protein-protein interactions and energy transfer. The NBS of functional resistance (R) genes and resistance gene analogs (RGA) was amplified from S. caripense genomic DNA via PCR using PCR primers. An overall high degree of sequence conservation was apparent throughout the P-loop, kinase-2 and kinase-3a motifs of NBS fragments from S. caripense and orthologous sequences of other plant species. Within an R gene, the NBS perhaps is the region of highest conservation, suggesting an essential role in triggering R protein activity. Within the non-TIR class of R genes a prominent sub-class similar to the potato R1 gene conferrring resistance to late blight, was discovered. The non-TIR-R1-like R gene fragments possessed 100% intact open reading frames, whereas 22% of all non-TIR-non-R1- fragments and 59% of all TIR-NBS RGA fragments had an interrupted reading frame or contained transposon specific sequence. Non-TIR-R1-like fragments obtained had high similarity to Solanaceae R genes and low similarity to RGAs of other plant species including A. thaliana and the cereals. It is concluded that R1-like NBS are specific for the Solanaceae and indicate a relatively recent evolutionary event.

Created from the Publication Database of the AIT Austrian Institute of Technology.