Publications in Scientific Journals:

E. Bojesen S, O. Ammerpohl, A. Weinhäusel, Haas Oskar Athur, H. Mettal, M. Bohle R, A. Borkhardt, U. Fuchs:
"Characterisation of the GRAF gene promoter and its methylation in patients with acute myeloid leukaemia and myelodysplastic syndrome";
British Journal of Cancer, 94 (2006), 323 - 332.



English abstract:
We report the isolation of the 5' flanking region of GRAF (GTPase regulator associated with the focal adhesion kinase), previously described as a putative tumour suppressor gene of acute myelogenous leukaemia and myelodysplastic syndrome, and demonstrate its promoter activity in reporter gene assays. Two putative protein-binding sites are identified of which one was sensitive to CpG methylation. The suppressed GRAF expression could be restored in leukaemia cell lines by treatment with a demethylating agent and an inhibitor of histone deacetylases. In contrast to normal tissues, which tested negative for GRAF promoter methylation, 11 of 29 (38%) bone marrow samples from patients with acute myeloid leukaemia or myelodysplastic syndrome were positive.

Keywords:
GRAF, AML, promoter methylation, MDS

Created from the Publication Database of the AIT Austrian Institute of Technology.