Publications in Scientific Journals:
Thomás Riedel, Frantisek Surman, S. Hageneder, O. Pop-Georgievski, Ch. Nöhammer, M. Hofner, Eduard Brynda, C. Rodriquez-Emmenegger, J. Dostalek:
"Hepatitis B plasmonic biosensor for the analysis of clinical serum samples";
Biosensors & Bioelectronics,
A plasmonic biosensor for rapid detection of protein biomarkers in complex media is reported. Clinical serum samples were analyzed by using a novel biointerface architecture based on poly[(N-(2-hydroxypropyl) methacrylamide)-co-(carboxybetaine methacrylamide)] brushes functionalized with bioreceptors. This biointerface provided an excellent resistance to fouling even after the functionalization and allowed for the first time the direct detection of antibodies against hepatitis B surface antigen (anti-HBs) in clinical serum samples using surface plasmon resonance (SPR). The fabricated SPR biosensor allowed discrimination of anti-HBs positive and negative clinical samples in 10 min. Results are validated by enzyme-linked immunoassays of the sera in a certified laboratory. The sensor could be regenerated by simple treatment with glycine buffer.
Surface plasmon resonance Biosensors, Polymer brushes, Antifouling
"Official" electronic version of the publication (accessed through its Digital Object Identifier - DOI)
Created from the Publication Database of the AIT Austrian Institute of Technology.