Publications in Scientific Journals:

Thomás Riedel, Frantisek Surman, S. Hageneder, O. Pop-Georgievski, Ch. Nöhammer, M. Hofner, Eduard Brynda, C. Rodriquez-Emmenegger, J. Dostalek:
"Hepatitis B plasmonic biosensor for the analysis of clinical serum samples";
Biosensors & Bioelectronics, 85 (2016), 85; 5 pages.



English abstract:
A plasmonic biosensor for rapid detection of protein biomarkers in complex media is reported. Clinical serum samples were analyzed by using a novel biointerface architecture based on poly[(N-(2-hydroxypropyl) methacrylamide)-co-(carboxybetaine methacrylamide)] brushes functionalized with bioreceptors. This biointerface provided an excellent resistance to fouling even after the functionalization and allowed for the first time the direct detection of antibodies against hepatitis B surface antigen (anti-HBs) in clinical serum samples using surface plasmon resonance (SPR). The fabricated SPR biosensor allowed discrimination of anti-HBs positive and negative clinical samples in 10 min. Results are validated by enzyme-linked immunoassays of the sera in a certified laboratory. The sensor could be regenerated by simple treatment with glycine buffer.

Keywords:
Surface plasmon resonance Biosensors, Polymer brushes, Antifouling


"Official" electronic version of the publication (accessed through its Digital Object Identifier - DOI)
http://dx.doi.org/10.1016/j.bios.2016.05.014


Created from the Publication Database of the AIT Austrian Institute of Technology.